Comparative Effects of L-histidine on the Activities of 5'-nucleotidase and Alkaline Phosphatase.

Amniotic fluid alkaline phosphatase, gamma-glutamyltransferase, and 5'-nucleotidase activity from 13 to 40 weeks' gestation, and alkaline phosphatase as an index of fetal lung maturity.

Reference ranges for amniotic fluid alkaline phosphatase, gamma-glutamyltransferase, and 5-nucleotidase are described from 13 to 40 weeks' gestation. Gamma-glutamyltransferase and 5-nucleotidase activities peak early in the second trimester and then decrease to low values. Alkaline phosphatase shows a similar pattern of activity from 13 to 29 weeks' gestation, but thereafter activity increases ...

Comparative study of serum 5' nucleotidase, alkaline phosphatase aminotransferases & bilirubin in alcoholic liver disease

Inhibition by concanavalin A as the basis for a specific assay of serum 5'-nucleotidase activity.

Concanavalin A inhibits serum 5'-nucleotidase activity, without causing significant inhibition of alkaline phosphatase activity. This observation serves as the basis for a new method for assaying the 5'-nucleotidase activity in serum, which depends upon the difference between the enzymic hydrolysis of adenosine-5'-monophosphate in the presence and absence of concanavalin A. A denosine released ...

Phosphatase activity in the ocular tissues.

This specific phosphatase is a 5-nucleotidase; it differs from the alkaline phosphatase in its range of action and in its optimal pH. Whereas alkaline phosphatase, which is non-specific, hydrolyses a great variety of phosphoric esters, the 5-nucleotidase hydrolyses only nucleotides with the phosphate group attached to the fifth carbon of the pentose, that is, muscle adenylic acid (adenosine-5-m...

Relationship between blood pressure of spontaneously hypertensive rats and alterations in membrane properties of mesenteric arteries.

Spontaneously hypertensive rats (SHR) and two strains of normotensive rats were compared with respect to enzymatic activities and calcium accumulation of plasma membrane and endoplasmic reticulum enriched fractions from their mesenteric arteries. Increased specific activities of alkaline phosphatase, 5'-nucleotidase and Mg2+-ATPase, and increased ATP-dependent calcium accumulation were found in...